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Typing of esterase D by isoelectric focusing
Author(s) -
Budowle Bruce
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050514
Subject(s) - isoelectric focusing , esterase , chromatography , resolution (logic) , isoelectric point , typing , polyacrylamide gel electrophoresis , polyacrylamide , electrophoresis , chemistry , thin layer , microbiology and biotechnology , layer (electronics) , analytical chemistry (journal) , biology , biochemistry , computer science , enzyme , genetics , artificial intelligence , organic chemistry
A method is described for obtaining reproducible resolution of Esterase D (EsD) phenotypes from human bloodstains by ultrathin‐layer polyacrylamide gel isoelectric focusing. By employing a pH 4.5–5.5 gradient and an electrode wick distance of 9.5 cm, all the common EsD variants were separated in 85 min. Electrofocusing of EsD took 53 and 60 min on the Cold Focus Apparatus and Ultrophor, respectively, when the distance separating the electrodes was 8.0 cm. Bloodstains up to 4 weeks old could be readily typed for EsD using this method.