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Effect of radioactive amino acids on chromosomes, viability and two‐dimensional protein patterns of cultured mammalian cells
Author(s) -
Zeindl Evelyn,
Klose Joachim
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050511
Subject(s) - amino acid , chinese hamster ovary cell , plating efficiency , incubation , biology , hela , cell culture , biochemistry , microbiology and biotechnology , protein biosynthesis , cell , genetics
The effect of 14 C‐labelled amino acids on three different established mammalian cell lines, i. e. , on Hep‐cells, HeLa‐cells, and Chinese hamster ovary cells, was studied. The cells were incubated in the presence of 20 μCi 14 C‐amino acids per 1 ml culture medium for 0–24 h or in the presence of 200 μCi/ml for 30 min. Thereafter, the chromosomes of the cells, the ability of the cells to form colonies (plating efficiency) and the protein patterns of the cells obtained by two‐dimensional electrophoresis were investigated. The results showed and increase in the number of chromosome breaks and a decrease in the plating efficiency when the incubation period was increased. In contrast, the frequency of qualitative and quantitative changes in the protein patterns of cells exposed to radioactive amino acids was not increased over the control level. Possible reasons for this discrepancy are discussed.