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pH gradient characteristics of commercially available carrier ampholytes and their uses for typing the human phosphoglucomutase locus (PGM 1 )
Author(s) -
Gill Peter,
Sutton John G.
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050505
Subject(s) - phosphoglucomutase , chromatography , chemistry , typing , locus (genetics) , analytical chemistry (journal) , biochemistry , biology , genetics , enzyme , gene
A number of different carrier ampholyte mixtures were compared in order to produce a pH gradient which separated the phosphoglucomutase‐1 (PGM 1 ) phenotypes most efficiently. Ultrathin gels incorporating a mixture of pH 5–7 and pH 6–8 Ampholine was shown to produce the best results. Application of samples to the cathodal end of the gel reduced the problem of overloading which is frequently encountered with ultrathin gels.