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Separation of the primary isoenzyme bands (a±, b±) determined by the phosphoglucomutase (PGM 1 ) locus on an immobilized pH gradient
Author(s) -
Sutton John G.,
Westwood Sara A.
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050414
Subject(s) - phosphoglucomutase , typing , locus (genetics) , isoelectric focusing , confusion , isozyme , chromatography , biology , genetics , microbiology and biotechnology , chemistry , biochemistry , enzyme , gene , psychology , psychoanalysis
This report describes a method of typing the phosphoglucomutase (PGM 1 ) locus (EC 2.7.5.1) isoenzymes from bloodstains on an immobilized pH gradient. A pH 5.8 to 6.8 gradient was prepared in which only the primary typing bands, a± and b±, associated with the 10 common PGM 1 locus phenotypes were separated. This system provides the forensic scientist with a method of typing anomalous results where confusion has arisen over the identity of the b+ and the c−/c+ isoenzymes observed by conventional electrofocusing. Successful typing of 2‐month‐old bloodstains was achieved within 2.0 h.