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Improved procedure for silanization of glass plates as supports for polyacrylamide gels
Author(s) -
Neuhoff Volker
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050413
Subject(s) - silanization , polyacrylamide , materials science , photopolymer , polymerization , composite material , chromatography , chemical engineering , polymer chemistry , chemistry , polymer , engineering
Abstract Silanization of plastic foils or glass plates is necessary for fixation of polyacrylamide gels to the support. When using glass plates as support, silanization creates problems which can be completely avoided when the silanized plates are heated for 30–45 min at 55–60°C prior to gel polymerization.