Double‐label autoradiography revisited: II. The  35 S and  75 Se‐system | Zendy

McConkey Edwin H. | Zendy; Anderson Catherine | Zendy

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Double‐label autoradiography revisited: II. The 35 S and 75 Se‐system

Author(s) -

McConkey Edwin H.,

Anderson Catherine

Publication year - 1984

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150050410

Subject(s) - selenium , physics , methionine , chemistry , organic chemistry , biochemistry , amino acid

The use of 75 Se in combination with 35 S for double‐label autoradiography has been quantitated. For gels 0.8 mm thick impregnated with EN 3 HANCE, the input ratio of 75 Se dpm/ 35 S dpm should be 3.3; and the second stage autoradiogram, if made with an interposed sheet of blackened XAR films as a screen to block 35 S, should be exposed 7.8x as long as the flourogram for SB film or 5.6 × as long as the fluorogram for XAR film. However, autoradiograms made under these conditions have larger, more diffuse spots than those made with the 3 H/ 35 S system and the cost of [ 75 Se]selenomethionine is much higher than the cost of [ 3 H]methionine.

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