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Determination of thiodiacetic acid in urine of people exposed to vinyl chloride by analytical capillary isotachophoresis
Author(s) -
Křiváková Ludmila,
Samcová Eva,
Boček Petr
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050408
Subject(s) - isotachophoresis , urine , chromatography , chemistry , vinyl chloride , reproducibility , vapours , capillary action , liter , chloride , biochemistry , organic chemistry , materials science , electrolyte , electrode , copolymer , neuroscience , composite material , biology , polymer , medicine
A method is introduced for the determination of thiodiacetic acid in urine by analytical capillary isotachophoresis with colunm coupling. Thiodiacetic acid is one of the final metabolites of carcinogenic vinyl chloride and appears at increased levels in the urine of people exposed to vinyl chloride vapours. The method enables the direct analysis of samples of 10 μl of urine without any pretreatment in about 45 min. The lowest detectable concentration of thiodiacetic acid was about 6 × 10 −6 mol/liter and the reproducibility of the analyses in the range of 2 ‐ 15 × 10 −5 mol. per liter was about 3 rel. %.