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Measurement of DNA superhelix density by dye titrationslab gel electrophoresis
Author(s) -
Poddar Saibal K.,
Maniloff Jack
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050312
Subject(s) - superhelix , ethidium bromide , gel electrophoresis of nucleic acids , gel electrophoresis , dna , electrophoresis , chemistry , chromatography , biochemistry , dna replication , dna supercoil
An ethidium bromide titration procedure using electrophoresis in horizontal multigels is described which facilitates measurement of DNA superhelix densities. Using this system, measured superhelix density was found to be dependent on the amount of DNA loaded on the gel.