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Fish species identification by electrophoresis of muscle proteins in sodium dodecyl sulfate‐containing polyacrylamide slab gels
Author(s) -
Corzo Javier,
RiolCimas José M.,
MeléndezHevia Enrique
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050310
Subject(s) - sodium dodecyl sulfate , myofibril , polyacrylamide gel electrophoresis , polyacrylamide , sodium , chromatography , gel electrophoresis , fish <actinopterygii> , sarcoplasm , chemistry , electrophoresis , biochemistry , biology , fishery , enzyme , endoplasmic reticulum , organic chemistry , polymer chemistry
Fish muscle proteins were separated by slab gel electrophoresis in 10–20 % polyacrylamide gradient gels in presence of sodium dodecyl sulfate. Three muscle extracts were tested: whole homogenate, sarcoplasmic and myofibrillar. Based on differences of the patterns in the 14 000–205 000 molecular weight range, identification of species of different orders, families and genus seems feasible. The most reproducible patterns were obtained with the whole homogenate. No changes were detected in the patterns after cold storage for several days (without freezing).