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Phosphoglucomutase‐1 subtyping of human bloodstains on ultrathin‐layer polyacrylamide gels
Author(s) -
Budowle Bruce
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050309
Subject(s) - phosphoglucomutase , isoelectric focusing , electrode , layer (electronics) , chromatography , subtyping , polyacrylamide gel electrophoresis , thin layer , electrophoresis , polyacrylamide , chemistry , materials science , analytical chemistry (journal) , biochemistry , polymer chemistry , nanotechnology , computer science , programming language , enzyme
A method is described for obtaining nondistorted, reproducible phosphoglucomutase‐1 subtyping patterns from fresh human bloodstains by ultrathin‐layer polyacrylamide gel isoelectric focusing. Electrofocusing of phosphoglucomutase on 0.2 mm thick gels took 52 and 80 min on the Cold Focus Apparatus and Ultrophor, respectively, when the distance separating the electrodes was 8.0 cm, whereas a 9.5 cm distance between electrode wicks required 95 min on the Multiphor to complete isoelectric focusing. The gels with 8.0 cm between electrode wicks provided sharper band patterns compared with the gels with 9.5 cm between electrode wicks.