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A preliminary evaluation of typing commonly occurring human erythrocyte acid phosphatase (ACP1) phenotypes in bloodstains, using the LKB Immobiline system
Author(s) -
Westwood Sara A.,
Sutton John G.
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050308
Subject(s) - isoelectric focusing , acid phosphatase , chemistry , chromatography , biochemistry , phosphatase , polyacrylamide gel electrophoresis , isozyme , typing , electrophoresis , phenotype , microbiology and biotechnology , enzyme , biology , gene
A preliminary study has been made of the use of immobilised pH gradients (Immobiline gels) for the separation of the six commonly occurring erythrocyte acid phosphatase (ACP1) phenotypes in blood lysates and stains. These pH gradients are immobilised in the matrix of the polyacrylamide gel and possess different properties from conventional isoelectric focusing (IEF) gels. A comparison was made between the profile of the ACP1 isoenzymes apparent on conventional IEF gels with that seen on the Immobiline gels.