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The effect of phytohemagglutinin on the electrophoretic mobility of purple membrane particles
Author(s) -
Kantcheva Maria R.,
Popdimitrova Nezabravka G.,
Stoylov Stoyl P.,
Kovatchev Dimiter E.
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050307
Subject(s) - electrophoresis , membrane , ionic strength , chemistry , lectin , biophysics , surface charge , phaseolus , erythrocyte membrane , chromatography , biochemistry , biology , botany , aqueous solution
Phytohemagglutinin (PHA)‐the lectin extracted from Phaseolus vulgaris ‐is found to induce electrophoretic mobility changes of purple membrane particles. The effect is dose‐dependent. The ionic strength of the suspending medium modifies the reduction of the membrane surface charge density.