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Cellulose acetate electrophoresis of glycosaminoglycans in the central nervous system
Author(s) -
Bertolotto Antonio,
Magrassi Maria L.
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050207
Subject(s) - glycosaminoglycan , dermatan sulfate , electrophoresis , heparan sulfate , heparin , chemistry , chromatography , chondroitin sulfate , cellulose acetate , biochemistry , cellulose
A new electrophoretic system, able to separate all the known standard glycosaminoglycans, was investigated as a method for the study of glycosaminoglycans extracted from tumours of the central nervous system. Densitometric analysis of standard glycosaminoglycan electrophoresis revealed high sesitivity and good repeatability. The electrophoretic system applied to biological samples based on a comigration of extracted glycosaminoglycans with standards, allowed subfractioning of chondroitinsulfates, dermatan sulfate and herapan sulfate and identifaction of heparan sulfate and heparin directly on the sheet by treatment with nitrous acid. Such characters permit the analysis of glycosaminoglycans present in small amount in biological samples.