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Artefacts associated with Nonidet P‐40 upon sodium dodecyl sulphate polyacrylamide gel electrophoresis of protein
Author(s) -
Marshall Thomas
Publication year - 1983
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150040614
Subject(s) - isoelectric focusing , polyacrylamide gel electrophoresis , electrophoresis , two dimensional gel electrophoresis , chemistry , chromatography , gel electrophoresis , sodium , gel electrophoresis of proteins , resolution (logic) , sodium dodecyl sulfate , polyacrylamide , streaking , biochemistry , biology , proteomics , enzyme , polymer chemistry , organic chemistry , artificial intelligence , computer science , microbiology and biotechnology , gene
One‐dimensional sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) of protein in the presence of Nonidet P–40 (NP–40) induces vertical streaking which breaks horizontal bands of protein to produce a “multiple spot” effect. The same effect can be induced upon high resolution two‐dimensional electrophoresis if the NP–40 concentration of the first‐dimension isoelectric focusing (IEF) gels is increased to 4 % w/v and the gels electrophoresed (SDS‐PAGE) without SDS‐equilibration. The relevance of this phenomenon as regards interpretation of two‐dimensional electrophoretic patterns is discussed.