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High resolution comparison of Plasmodium knowlesi clones of different variant antigen phenotypes by two‐dimensional gel electrophoresis and computer analysis
Author(s) -
Howard Russel J.,
Aley Stephen B.,
Lemkin Peter F.
Publication year - 1983
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150040610
Subject(s) - plasmodium knowlesi , biology , antigenic variation , antigen , clone (java method) , microbiology and biotechnology , phenotype , gel electrophoresis , virology , gene , genetics , plasmodium falciparum , immunology , plasmodium vivax , malaria
Antigenic variation of the schizont‐infected cell agglutination‐antigen on erythrocytes infected by Plasmodium knowlesi is due to the expression of different malarial proteins. Here we examined whether antigenic variation is accompanied by multiple phenotypic changes in the proteins synthesized by P. knowlesi. The malarial proteins of two clones, one of which was produced by antigenic variation from the other clone in vivo , were labeled by [ 3 H]isoleucine uptake during in vitro parasite growth. The proteins were separated by two‐dimensional gel electrophoresis, detected by fluorography and compared using the GELLAB computer system. In the molecular weight range 35 000 to 230 000 and pH range 4.55 to 6.10, we found only four qualitative differences and two robust quantitative differences among approximately 500 proteins characterizing the protein phenotype of these clones. One clone exhibited four minor protein spots absent from the other. We conclude that antigenic variation in P. knowlesi represents the differential expression of a very small number of malarial genes.