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An improved and simplified apparatus for protein extraction and concentration from gel slices, using moving boundary electrophoresis
Author(s) -
Lan Birgit An Der,
Horuk Richard,
Sullivan James V.,
Chrambach Andreas
Publication year - 1983
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150040505
Subject(s) - chromatography , gel electrophoresis , electrophoresis , sodium dodecyl sulfate , chemistry , bovine serum albumin , extraction (chemistry) , free flow electrophoresis , buffer (optical fiber) , polyacrylamide gel electrophoresis , two dimensional gel electrophoresis , albumin , pulsed field gel electrophoresis , gel electrophoresis of proteins , biochemistry , computer science , enzyme , proteomics , telecommunications , genotype , gene
A previous design of an apparatus for extraction and concentration of proteins from gel slices by moving boundary electrophoresis has been simplified, making it into an upper buffer reservoir accessory for the multifunctional gel tube apparatus commonly used for gel electrophoresis. The design of the collection cup has been improved to facilitate assembly and thereby reduce the risk of leakage. The performance of the apparatus was evaluated by the isolation from sodium dodecyl sulfate ‐ polyacrylamide gel electrophoresis of radiolabeled bovine serum albumin and glucagon receptor.