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Separation of legumin and vicilin, the storage proteins from germinating Vicia faba seeds, by electrophoresis on cellulose acetate membranes
Author(s) -
Leslie Philip,
Bonner Richard,
Vandevalle Ian,
Olsson Richard
Publication year - 1983
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150040415
Subject(s) - vicilin , legumin , vicia faba , isoelectric point , chromatography , storage protein , electrophoresis , isoelectric focusing , chemistry , cellulose acetate , membrane , biochemistry , biology , botany , gene , enzyme
Conventional electrophoretic methods of assessing the purity of a protein are both expensive and laborious. The method of zonal isoelectric precipitation electrophoresis involves precipitation of the protein under investigation at its isoelectric point after application onto cellulose acetate membranes. Contaminating proteins will be mobile and are easily visualized by conventional staining methods. Application of zonal isoelectric precipitation electrophoresis to the storage proteins of Vicia faba seeds (legumin and vicilin) has proved valuable in this laboratory, and, by adjustment of the buffering system, can be made to work for other protein mixtures.