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Subtyping of human red cell phosphoglucomutase (PGM 1 ) using conventional agarose gel electrophoresis
Author(s) -
Bäur Walter,
Dissing Jørgen
Publication year - 1983
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150040407
Subject(s) - phosphoglucomutase , subtyping , electrophoresis , agarose , isoelectric focusing , chemistry , chromatography , affinity electrophoresis , isozyme , agarose gel electrophoresis , biochemistry , enzyme , dna , affinity chromatography , computer science , programming language
Abstract A simple, rapid electrophoretic method for the subtyping of phosphoglucomutase (PGM 1 ) isozymes is presented. Electrophoresis was performed in an agarose gel containing an acetic acid/L‐histidine/citric acid buffer system at pH 5.30 for 2.5 h. The separation of the isozymes was at least as good as that obtained by isoelectric focusing, but in contrast with that method, the electrophoretic procedure did not require expensive equipment or carrier ampholytes.