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Agarose gel electrophoresis of bacteriophages and related particles. IV. An improved procedure for determining the size of spherical particles
Author(s) -
Serwer Philip,
Allen Jerry L.
Publication year - 1983
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150040405
Subject(s) - agarose , radius , electrophoresis , particle size , particle (ecology) , agarose gel electrophoresis , colloid , colloidal particle , hydrodynamic radius , effective radius , chromatography , chemistry , analytical chemistry (journal) , materials science , physics , nanotechnology , nanoparticle , dna , dynamic light scattering , biology , ecology , biochemistry , computer security , quantum mechanics , galaxy , computer science
Attempts have been made to improve the accuracy with which sieving during electrophoresis in gels can be used to determine the size of viruses and related particles. Using as samples spherical bacteriophages and related particles of known radius (13.3–41.9 nm), it was found that electrophoretic mobility (μ) was a linear function of agarose concentration ( A ) in a hydroxyethylated agarose (SeaPlaque, Marine Colloids) for 0.4 ⩽ A ⩽ 1.6. The value of A extrapolated to a μ of 0 ( A 0 ) was a decreasing function of particle outer radius and was independent of the solid support‐free μ. Procedures for determining outer particle radius from values of A 0 have been developed. When used with internal standards, these procedures measure outer particle radius with an accuracy of ±4 %.