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Nonspecific binding of p‐phenylenediamine‐pyrocatechol peroxidase substrate to electrofocused proteins
Author(s) -
Cantarow Walter D.,
Saravis Calvin A.,
Sampson Christian E.
Publication year - 1983
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150040212
Subject(s) - peroxidase , substrate (aquarium) , chemistry , reagent , chromatography , nuclear chemistry , biochemistry , enzyme , organic chemistry , biology , ecology
Nonspecific staining of electrofocused protein bands was found with the peroxidase product of Hanker‐Yates reagent (a mixture of p‐phenylenediamine and pyrocatechol). No difficulty was encountered with diaminobenzidine as peroxidase substrate.