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Two‐dimensional polyacrylamide gel electrophoresis in micro‐gels: Gradient gel electrophoresis followed by dodecyl sulfate gel electrophoresis
Author(s) -
Beltle Wolfgang,
Zimmermann Sabine,
Bopp Martin,
Murach KarlFriedrich
Publication year - 1983
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150040208
Subject(s) - gel electrophoresis of proteins , chromatography , electrophoresis , gel electrophoresis , molecular weight size marker , polyacrylamide , sodium dodecyl sulfate , chemistry , polyacrylamide gel electrophoresis , gel electrophoresis of nucleic acids , free flow electrophoresis , cuvette , two dimensional gel electrophoresis , biochemistry , polymer chemistry , optics , physics , proteomics , gene , enzyme
A miniature system for two‐dimensional polyacrylamide gel electrophoresis is presented. Discontinuous sodium dodecyl sulfate gel electrophoresis in micro‐slab gels following high resolution micro‐electrophoresis in cylindrical continuous polyacrylamide gradient gels separates macromolecules after an initial size fractionation into their subunits. The construction of a glass cuvette for micro‐slab gels and of a suitable electrophoresis tank is described in detail. Furthermore, procedures for silver staining and fluorography initially developed for gels of normal size were adapted to micro‐slab gels. Crude tissue extracts of Funaria hygrometrica L. Sibth. were analyzed without any further purfication. Twenty samples can be processed in about eight hours.