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Detection of bacteriophage‐antibody complexes by agarose gel electrophoresis
Author(s) -
Serwer Philip,
Hayes Shirley J.
Publication year - 1982
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150030603
Subject(s) - agarose , antiserum , bacteriophage , agarose gel electrophoresis , electrophoresis , gel electrophoresis , antibody , microbiology and biotechnology , chemistry , color marker , gel electrophoresis of nucleic acids , chromatography , molecular weight size marker , antigen , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , biology , dna , biochemistry , escherichia coli , enzyme , gene , immunology
To detect bacteriophage‐antibody complexes, specific antiserum has been incubated with bacteriophages and antiserum‐induced aggregation has been detected by agarose gel electrophoresis, using differential sieving of the gel to fractionate aggregates by size. Agarose gel electrophoresis should also be of use in monitoring reaction of other virus‐sized antigens with antibodies.