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Electrophoretic separation of human lymphocytes by means of immunomicrospheres
Author(s) -
Smolka Adam,
Kempner David,
Rembaum Alan
Publication year - 1982
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150030510
Subject(s) - electrophoresis , free flow electrophoresis , fraction (chemistry) , chemistry , peripheral blood , lymphocyte , chromatography , cell , microbiology and biotechnology , biology , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , biochemistry , immunology , enzyme
Abstract Immuno‐labeling of human peripheral blood lymphocytes with either polyglutaraldehyde or polyacrolein microspheres was shown by analytical electrophoresis to generate two electrokinetically distinct sub‐populations of lymphocytes. By means of free‐flow electrophoresis, the high mobility fraction was found to consist predominantly of T lymphocytes using an anti‐T cell antibody, while the low mobility fraction consisted of lymphocytes labeled with immunomicrospheres, identifying these cells as B lymphocytes. The immunomicrosphere‐induced reduction of B‐cell mobility below that of the T cells thus allowed the enrichment and recovery of viable T cells in preparative quantities by free‐flow electrophoresis.