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Rapid purification of antibodies to single band purity using recycling isoelectric focusing
Author(s) -
Binion Steven B.,
Rodkey L. Scott,
Egen Ned B.,
Bier Milan
Publication year - 1982
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150030507
Subject(s) - isoelectric focusing , antibody , monoclonal antibody , chromatography , flexibility (engineering) , population , chemistry , isoelectric point , biochemistry , biology , enzyme , immunology , statistics , demography , mathematics , sociology
Abstract Recycling isoelectric focusing (RIEF) was used to isolate monoclonal antibodies from an electrophoretically heterogeneous rabbit antibody population. Milligram quantities of antibodies were processed to single band purity in 4 h. The ease of operation, extremely short run times, and high resolution of the RIEF apparatus give this system great flexibility. The demonstrated capacity of the RIEF system to process large quantities of protein suggests that the system is applicable to the largescale purification of antibodies.