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Isotachophoretic determination of a relative apparent mobility as an estimate of the electrophoretic mobility of ions
Author(s) -
Carchon Hubert,
Eggermont Ephrem
Publication year - 1982
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150030505
Subject(s) - isotachophoresis , electrophoresis , ion , chemistry , chromatography , ionic bonding , function (biology) , analytical chemistry (journal) , electrolyte , organic chemistry , electrode , evolutionary biology , biology
An isotachophoretic procedure for the estimation of the electrophoretic mobility of ions is described. The parameter, called apparent mobility (AM), is a function of the conditions of assay. Defining a relative apparent mobility (RAM) as the percentage value of the AM of the ion studied and the AM of a fully dissociated reference substance, an operational estimate of the electrophoretic mobility of ionic species is obtained. RAM values, therefore, are only a function of the leading ion and of the ion studied. If this ion demonstrates acid‐base equilibria, its RAM value will become pH dependent. As RAM values are independent on the conditions of assay, classification is meaningful. For a number of anions, RAM values may help to find out the optimal conditions for separation of ions by analytical isotachophoresis.