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Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms
Author(s) -
Yakin Habib M.,
Kronberg Harald,
Zimmer HansG.,
Neuhoff Volker
Publication year - 1982
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150030503
Subject(s) - electropherogram , densitometer , smoothing , piecewise linear function , gaussian , computer science , set (abstract data type) , algorithm , pattern recognition (psychology) , biological system , artificial intelligence , mathematics , electrophoresis , computer vision , optics , chromatography , chemistry , physics , mathematical analysis , biology , computational chemistry , programming language
In order to increase accuracy and precision of quantifying one‐dimensional electropherograms by digital signal processing, a set of algorithms is presented that operate on the optical density values acquired by a linear scanning densitometer. These operations include smoothing of the measuring values, background correction by an optimized piecewise linear baseline, and integration of the peak areas after two‐sided fit of half‐Gaussian profiles to each stained protein band. The algorithms are implemented as BASIC‐programs and are readily adaptable to a variety of electrophoretic separations. The procedure has been applied successfully in research [1] as well as in clinical evaluations [2].