Premium
Sensitive fluorescence staining for RNA and DNA species separated in polyacrylamide gels
Author(s) -
Neuhoff Volker,
Huether Gerald,
Engelhardt Uta
Publication year - 1982
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150030312
Subject(s) - staining , polyacrylamide , nucleic acid , fluorescence , rna , dna , chemistry , polyacrylamide gel electrophoresis , nucleic acid quantitation , chromatography , fluorescent staining , microbiology and biotechnology , biochemistry , biology , enzyme , gene , genetics , physics , quantum mechanics
The fluorescent dye, Hoechst Dye No. 33258, was found to be of excellent quality for staining of not only DNA, as reported in the literature, but also of RNA species after separation in polyacrylamide micro gels. The staining procedure is simple and very sensitive. There are no problems with background staining and under certain conditions the nucleotide‐dye complex is stable for a long time. The fluorescence intensity is linear to the amount of nucleic acid bound by the dye within certain limits.