Isoelectric focusing of α 1 ‐antitrypsin (PI) using restricted pH‐range carrier ampholytes in combination with a highly cross‐linked gel and a separator | Zendy

Klasen Eduard C. | Zendy; Rigutti Adriana | Zendy

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Isoelectric focusing of α 1 ‐antitrypsin (PI) using restricted pH‐range carrier ampholytes in combination with a highly cross‐linked gel and a separator

Author(s) -

Klasen Eduard C.,

Rigutti Adriana

Publication year - 1982

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150030311

Subject(s) - isoelectric focusing , pi , chemistry , chromatography , separator (oil production) , immobilized ph gradient , polyacrylamide gel electrophoresis , analytical chemistry (journal) , biochemistry , physics , thermodynamics , enzyme

A simple technique for polyacrylamide gel isoelectric focusing of α 1 ‐antitrypsin (PI) using thin, highly cross‐linked gels, restricted pH‐range carrier ampholytes and a separator was developed. This method enables easy typing of all known PI phenotypes including the four PI*M subtypes.

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