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Isoelectric focusing of α 1 ‐antitrypsin (PI) using restricted pH‐range carrier ampholytes in combination with a highly cross‐linked gel and a separator
Author(s) -
Klasen Eduard C.,
Rigutti Adriana
Publication year - 1982
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150030311
Subject(s) - isoelectric focusing , pi , chemistry , chromatography , separator (oil production) , immobilized ph gradient , polyacrylamide gel electrophoresis , analytical chemistry (journal) , biochemistry , physics , thermodynamics , enzyme
A simple technique for polyacrylamide gel isoelectric focusing of α 1 ‐antitrypsin (PI) using thin, highly cross‐linked gels, restricted pH‐range carrier ampholytes and a separator was developed. This method enables easy typing of all known PI phenotypes including the four PI*M subtypes.