Routine phenotyping of phosphoglucomutase (PGM 1 ) by thin‐layer focusing: Isoelectric points of 14 different variants | Zendy

Dykes Dale D. | Zendy; Copouls Barbara A. | Zendy; Polesky Herbert F. | Zendy

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Routine phenotyping of phosphoglucomutase (PGM 1 ) by thin‐layer focusing: Isoelectric points of 14 different variants

Author(s) -

Dykes Dale D.,

Copouls Barbara A.,

Polesky Herbert F.

Publication year - 1982

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150030310

Subject(s) - isoelectric focusing , phosphoglucomutase , agarose , thin layer , isoelectric point , chromatography , resolution (logic) , layer (electronics) , biology , chemistry , biochemistry , enzyme , computer science , artificial intelligence , organic chemistry

A routine technique for phenotyping the PGM 1 marker system was developed using thin‐layer isoelectric focusing on agarose. Resolution of PGM 1 variants by thin‐layer isoelectric focusing on 0.5 mm gels was found to be superior to isoelectric focusing on ⩾ 1 mm gels. Isoelectric points were determined for 14 different PGM 1 variants and should serve as a useful tool for comparing variants between laboratories.

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