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Routine phenotyping of phosphoglucomutase (PGM 1 ) by thin‐layer focusing: Isoelectric points of 14 different variants
Author(s) -
Dykes Dale D.,
Copouls Barbara A.,
Polesky Herbert F.
Publication year - 1982
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150030310
Subject(s) - isoelectric focusing , phosphoglucomutase , agarose , thin layer , isoelectric point , chromatography , resolution (logic) , layer (electronics) , biology , chemistry , biochemistry , enzyme , computer science , artificial intelligence , organic chemistry
A routine technique for phenotyping the PGM 1 marker system was developed using thin‐layer isoelectric focusing on agarose. Resolution of PGM 1 variants by thin‐layer isoelectric focusing on 0.5 mm gels was found to be superior to isoelectric focusing on ⩾ 1 mm gels. Isoelectric points were determined for 14 different PGM 1 variants and should serve as a useful tool for comparing variants between laboratories.