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Electrophoretic cell separation using polyacrolein microspheres
Author(s) -
Kempner David H.,
Smolka Adam J. K.,
Rembaum Alan
Publication year - 1982
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150030210
Subject(s) - electrophoresis , microsphere , chemistry , chromatography , methacrylic acid , acrolein , cell , biochemistry , chemical engineering , catalysis , organic chemistry , polymer , copolymer , engineering
The electrophoretic mobility of a number of microspheres of homo‐ or copolymerized acrolein has been studied. It was found that increasing amounts of methacrylic acid increased the negative mobility of the microspheres, whereas reaction of the microspheres with 1,6‐diaminohexane decreased the negative mobility. When coated with antibody and specifically bound to cells, these microspheres were found to alter the target cells' electrophoretic mobility. This process allowed the separation of synthetic mixtures of microsphere‐labeled and unlabeled cell populations. Over 90 % of the recovered cells were found in fractions that were essentially either pure labeled or unlabeled cells.