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Agarose gel electrophoresis of bacteriophages and related particles. II. Correction of electrophoretic mobilities for the electro‐osmosis of agarose
Author(s) -
Serwer Philip,
Hayes Shirley J.
Publication year - 1982
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150030204
Subject(s) - agarose , electrophoresis , chromatography , bacteriophage , chemistry , capillary electrophoresis , electro osmosis , biochemistry , escherichia coli , gene
Bacteriophage T4, bacteriophage T4 without its tail fibers, and bacteriophage P22 without its tail have been subjected to electrophoresis in agarose gels; electrophoretic mobility (μ) of these particles as a function of the concentration of agarose ( A ) has been determined using preparations of agarose with different amounts of electro‐osmosis. When μ was extrapolated to an A of 0 (μ o ), it was found that a term, μ E , constant for each preparation of agarose must be added to μ o to correct for electro‐osmotic flow. A relationship between μ E and a manufacturer‐determined coefficient of electro‐osmosis has been empirically derived. Corrected μ o 's are in agreement with data obtained by solid support‐free electrophoresis.