Agarose gel electrophoresis of bacteriophages and related particles. II. Correction of electrophoretic mobilities for the electro‐osmosis of agarose | Zendy

Serwer Philip | Zendy; Hayes Shirley J. | Zendy

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Agarose gel electrophoresis of bacteriophages and related particles. II. Correction of electrophoretic mobilities for the electro‐osmosis of agarose

Author(s) -

Serwer Philip,

Hayes Shirley J.

Publication year - 1982

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150030204

Subject(s) - agarose , electrophoresis , chromatography , bacteriophage , chemistry , capillary electrophoresis , electro osmosis , biochemistry , escherichia coli , gene

Bacteriophage T4, bacteriophage T4 without its tail fibers, and bacteriophage P22 without its tail have been subjected to electrophoresis in agarose gels; electrophoretic mobility (μ) of these particles as a function of the concentration of agarose ( A ) has been determined using preparations of agarose with different amounts of electro‐osmosis. When μ was extrapolated to an A of 0 (μ o ), it was found that a term, μ E , constant for each preparation of agarose must be added to μ o to correct for electro‐osmotic flow. A relationship between μ E and a manufacturer‐determined coefficient of electro‐osmosis has been empirically derived. Corrected μ o 's are in agreement with data obtained by solid support‐free electrophoresis.

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