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High resolution ultrathin‐layer isoelectric focusing of PGM 1 ‐subgroups in forensic blood typing
Author(s) -
Pflug Werner,
De La Vigne Ulf,
Bruder Walter
Publication year - 1981
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150020514
Subject(s) - isoelectric focusing , chromatography , typing , analytical chemistry (journal) , resolution (logic) , thin layer , chemistry , polyacrylamide , electrophoresis , isozyme , high resolution , polyacrylamide gel electrophoresis , layer (electronics) , materials science , biology , biochemistry , computer science , genetics , artificial intelligence , enzyme , polymer chemistry , remote sensing , organic chemistry , geology
Ultrathin‐layer isoelectric focusing in 100 um polyacrylamide gels is described for the separation of the isozymes of the PGM 1 ‐locus. The PGM1‐subgroups are best resolved in pH 5–7 gradients obtained by mixing Ampholine and Servalyt carrier ampholytes in a 2:1 ratio. By using a high field strength (180 V/cm) in the final stage of isoelectric focusing, the isozyme bands are sharper than in conventional thin‐layer gels. This facilitates identification of the subgroups. Distortions of the pH gradient are avoided if low initial field strengths (15–20 V/cm) are used. The method is highly reproducible and equally applicable for the analysis of blood samples as well as blood stains.