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Silver Stains for proteins in polyacerylamide gels: A comparison of six methods
Author(s) -
Ochs Denis C.,
McConkey Edwin H.,
Sammons David W.
Publication year - 1981
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150020509
Subject(s) - staining , silver stain , polyacrylamide , chromatography , chemistry , electrophoresis , polyacrylamide gel electrophoresis , microbiology and biotechnology , biology , biochemistry , polymer chemistry , genetics , enzyme
Six of the most recent silver staining method for detecting polypeptides on polyacrylamide gels were compared. We found the method of Sammons et al. ( Electrophoresis , 1981, 2, 135–141) to be least expensive and most reproducible, For staining gels of 1.5 mm in thickness, it is also the most sensitive method. Other methods may be preferable for staining gels 0.8 mm or less in thickness.