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The fabrication of multichannel tubing and its use in a continuous‐flow isielectric focusing apparatus to give smooth laminar flow
Author(s) -
Quarmby Christopher
Publication year - 1981
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150020403
Subject(s) - laminar flow , peristaltic pump , materials science , flow (mathematics) , manifold (fluid mechanics) , volumetric flow rate , fabrication , mechanics , mechanical engineering , physics , engineering , medicine , alternative medicine , pathology
Details are given of a method of casting multichannel tubing in silicone rubber in the form of a slab 2 mm thick and up to 380 mm long. Up to 264 parallel channels were formed at 0.8 mm centers along the length of the material, each channel being 0.4 mm in diameter. The material was used both as the outlet manifold in an apparatus for continuous‐flow isoelectric focusing (IEF), the construction of which is described, and also in conjunction with a slow‐running peristaltic pump as multichannel pump‐tubing when it was found that at a flow‐rate of about 0.3 ml h −1 per channel, the variation between channels was less than 0.6%. The coupling of the pump‐tubing to the outlet manifold of the separation cell of the IEF apparatus resulted in a very smooth laminar flow of liquid in the separation cell itself.

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