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The nature of observed schlieren patterns in isoelectric focusing gels and their use for position of banded proteins
Author(s) -
Edwards Jesse J.,
Anderson Norman G.
Publication year - 1981
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150020307
Subject(s) - isoelectric focusing , classification of discontinuities , schlieren , position (finance) , characterization (materials science) , isoelectric point , materials science , simple (philosophy) , chromatography , chemistry , biological system , optics , physics , nanotechnology , biology , biochemistry , mathematics , philosophy , finance , epistemology , economics , enzyme , mathematical analysis
A simple and versatile optical technique for viewing discontinuities in the thickness of horizontal isoelectric focusing gels is described. The discontinuities in the gel are reproducible and relate directly to the ampholyte distribution. Characterization of the capture of the discontinuities in the gel is presented as well as a demonstration of how they may used for localization of focused proteins.