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Analysis of bacteriophage T4 coat proteins by two‐dimensional electrophoresis and computerized densitometry
Author(s) -
Gersten Dogulas M.,
Kurian Ponnamma,
Ledly Gary,
Park Chan M.,
Suhocki Paul V.
Publication year - 1981
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150020210
Subject(s) - densitometry , coomassie brilliant blue , electrophoresis , chromatography , bacteriophage , sodium dodecyl sulfate , chemistry , gel electrophoresis , resolution (logic) , staining , coat protein , microbiology and biotechnology , mass spectrometry , biochemistry , biology , escherichia coli , rna , genetics , artificial intelligence , gene , computer science , physics , quantum mechanics
We have designed treatment conditions which allow analysis of the coat proteins of bacteriophage T4D+ by high resolution two‐dimensional electrophoresis. The best results were obtained when the mature phage particles were subjected to hypertoniclysis and DNase treatment before solubilization with sodium dodecyl sulfate (SDS). Samples containing 250 ug protein gave the optimum resolution, producing 45 spots as visualized by Coomassie Blue staining. Computerized densitometry measurements showed that these proteins range in relative abundance from less than 0.22% to 11.8% of the total.