Characterization of mouse lymphocytes by analytical automatic cell electrophoresis

Mouse splenic lymphocytes were examined for electrophoretic mobility by an automatic cell electrophoresis. The whole population of lymphocytes showed a bimodal histogram of electrophoretic mobility. In contrast, adherent cells to a nylon wool column (B cell‐rich) and nonadherent cells treated with anti‐mouse IgG and complement (T cell‐rich) gave a unimodal histogram. A linear relationship existed between the electrophoretic mobility of mixture of T and B cells and their ratio detected by the method of immunofluorescence, suggesting that the ratio of T to B cells can easily be determined from the electrophoretic mobility of lymphocytes.