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Assay of UDP‐glucuronyltransferease with mixtures of acceptors using analytical capillary isotachophoresis
Author(s) -
Holloway Christopher J.,
HusmannHolloway Sabine,
Brunner Gorig
Publication year - 1981
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150020104
Subject(s) - isotachophoresis , glucuronidation , chemistry , chromatography , substrate (aquarium) , glucuronic acid , microsome , enzyme , biochemistry , electrolyte , polysaccharide , electrode , geology , oceanography
Abstract Analytical capillary isotachophoresis has been applied as an analytical tool for the assay of the microsomal enzyme UDP‐glucuronyltransferase, which is an important system for the detoxification of drugs and many endogenous and exogenous toxins. The substrate donor molecule UDP‐glucuronic acid, various products of hydrolytic cleavage, and the products of glucuronidation β‐glucuronides, can be assayed in a single run. Furthermore, the glucuronidation of mixtures of acceptors can be monitored simultaneously, since the various β‐glucuronides are separated from one another. This method offers a more comprehensive assay of glucuronidation reactions than has been possible using previously published procedures.