Premium
Purification of pepsinogen I from human urine by means of DEAE‐chromatography and isotachophoresis
Author(s) -
Axelsson Christen Kirk,
Axelsen Nils Holger,
Svendsen Per Just
Publication year - 1980
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150010308
Subject(s) - isotachophoresis , chromatography , chemistry , immunoelectrophoresis , urine , elution , sephadex , biochemistry , enzyme , antibody , biology , electrode , electrolyte , immunology
Abstract The present paper gives a description of a procedure for isolation of pepsinogen I from human urine. The initial steps are concentration by batch adsorption to DEAE‐Sephadex, desorption by addition of NaCl, and DEAE‐chromatography. The final step is preparative isotachophoresis with Ampholine carrier ampholytes as spacers. Exact visualization of the fractionations is obtained by fused rocket immunoelectrophoresis. The procedure provides pepsinogen I of high purity as shown by crossed immunoelectrophoresis, agar gel electrophoresis and N‐terminal aminoacid analysis. The yield of the procedure is 0.05–0.1 mg pepsinogen I per liter urine.