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pH Gradient flattening in isoelectric focusing in long polyacrylamide gels
Author(s) -
Rapaport Ray N.,
Jackiw Andrew,
Brown Ray K.
Publication year - 1980
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150010210
Subject(s) - isoelectric focusing , polyacrylamide , chemistry , flattening , chromatography , resolution (logic) , immobilized ph gradient , analytical chemistry (journal) , separator (oil production) , materials science , polymer chemistry , computer science , biochemistry , physics , thermodynamics , artificial intelligence , composite material , enzyme
Isoelectric focusing in 60 cm long gels improves resolution in multiple‐component systems by providing very shallow, linear pH gradients. Broad range ampholytes available commercially or synthesized in our laboratory develop pH gradients of 0.1 pH/cm at voltages of 1500 to 4500 V, with running times of 25–30 h. Shallower gradients result with narrow range ampholytes or separator isoelectric focusing. Hemoglobin, human serum albumin, and an α‐N‐acetylgalactosaminidase have been used for defining this system.