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Principles and instrumentation of analytical cell electrophoresis
Author(s) -
Catsimpoolas Nicholas
Publication year - 1980
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150010202
Subject(s) - instrumentation (computer programming) , rotation (mathematics) , capillary action , electrophoresis , laser doppler velocimetry , optics , materials science , computer science , chemistry , physics , chromatography , artificial intelligence , medicine , blood flow , composite material , operating system
Measurement of the electrophoretic mobility of cells can be performed with various types of apparatus. Basically, these instruments can be classified into two broad categories i.e. those where physical separation does not take place and those which actually separate cell populations according to their surface charge. The former category includes the microscopic/image analysis method and the laser light scattering Doppler velocimetry apparatus. These two types of instruments can be used only for analytical purposes. Physical separation combined with analytical capabilities requires some form of anti‐convection stabilization of the separated species. This can be accomplished by capillary action, mechanical or electromagnetic rotation, and flowing or stationary density gradients. Several instruments exist which utilize each of the above principles. Monitoring of the separation is usually performed by optical scanning.