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Flattening, shifting and comparing pH gradients in slab gel electrofocusing by local increases of gel volume
Author(s) -
Altland Klaus,
Kaempfer Michael
Publication year - 1980
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150010110
Subject(s) - flattening , isoelectric focusing , slab , chemistry , volume (thermodynamics) , chromatography , strips , analytical chemistry (journal) , materials science , composite material , geology , physics , thermodynamics , biochemistry , geophysics , enzyme
A simple and versatile technique of horizontal slab gel electrofocusing is described which is based on a discontinuity of the gel volume along the pH axis achieved by overlaying ampholyte containing gel strips, of a defined volume and shape, at various locations on a base gel during the prerun of isoelectric focusing. In the electric field the overlaid gel strip delivers ampholytes of a distinct pI interval into the strip‐free part of the base gel and removes from the latter some ampholytes present in the system. Flattening of the pH gradient in the strip‐free part of the gel is the consequence. The technique is especially useful for thin gels and can be performed with any commercially available horizontal slab apparatus.