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Assembling a Low‐volume Biofuel Cell on a Screen‐printed Electrode for Glucose Sensing
Author(s) -
Becker Jana M.,
Lielpetere Anna,
Szczesny Julian,
Ruff Adrian,
Conzuelo Felipe,
Schuhmann Wolfgang
Publication year - 2022
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202200084
Subject(s) - bilirubin oxidase , glucose oxidase , electrode , gas diffusion electrode , biosensor , chemistry , redox , pyrroloquinoline quinone , materials science , nanotechnology , electrochemistry , inorganic chemistry , biochemistry , enzyme , cofactor
An enzymatic biofuel cell is integrated on a screen‐printed electrode as a basis for a self‐powered biosensor. A glucose/O 2 biofuel cell consisting of a pyrroloquinoline quinone‐dependent glucose dehydrogenase embedded within an Os‐complex modified redox polymer bioanode to oxidize glucose and a non‐limiting bilirubin oxidase‐based gas diffusion biocathode in the direct‐electron transfer regime for the reduction of O 2 showed a glucose‐dependent current and power output. For full integration on a single screen‐printed electrode, a miniaturized agar salt bridge was introduced between the two bioelectrodes to ensure operation of the assembly in a two‐compartment configuration with each electrode operating at optimal conditions.

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