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Development of a Temperature‐pulse Enhanced Electrochemical Glucose Biosensor and Characterization of its Stability via Scanning Electrochemical Microscopy
Author(s) -
Wert Stefan,
Iken Heiko,
Schöning Michael J.,
Matysik FrankMichael
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202100089
Subject(s) - glucose oxidase , amperometry , biosensor , electrochemistry , scanning electrochemical microscopy , materials science , thermal stability , chemical engineering , microscopy , scanning electron microscope , analytical chemistry (journal) , electrode , chemistry , nanotechnology , chromatography , composite material , optics , physics , engineering
Glucose oxidase (GOx) is an enzyme frequently used in glucose biosensors. As increased temperatures can enhance the performance of electrochemical sensors, we investigated the impact of temperature pulses on GOx that was drop‐coated on flattened Pt microwires. The wires were heated by an alternating current. The sensitivity towards glucose and the temperature stability of GOx was investigated by amperometry. An up to 22‐fold increase of sensitivity was observed. Spatially resolved enzyme activity changes were investigated via scanning electrochemical microscopy. The application of short (<100 ms) heat pulses was associated with less thermal inactivation of the immobilized GOx than long‐term heating.