Premium
Sensitive and Low‐background Electrochemical Immunosensor Employing Glucose Dehydrogenase and 1,10‐Phenanthroline‐5,6‐dione
Author(s) -
Jiaul Haque AlMonsur,
Kwon Jungwook,
Kim Jihyeon,
Kim Gyeongho,
Lee NamSihk,
Ho Yoon Young,
Yang Haesik
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202100079
Subject(s) - redox , electrochemistry , dehydrogenase , detection limit , chemistry , flavin adenine dinucleotide , phenanthroline , nuclear chemistry , inorganic chemistry , enzyme , chromatography , electrode , biochemistry , cofactor
We report a sensitive electrochemical immunosensor with a low electrochemical background level, which results from electrochemical‐enzymatic (EN) redox cycling based on mediated electrochemical oxidation of an electro‐inactive reductant (glucose) at 0.0 V. The EN redox cycling employs flavin adenine dinucleotide‐dependent glucose dehydrogenase and 1,10‐phenanthroline‐5,6‐dione (PD). When PD was compared with five common quinone‐based electron mediators, PD enabled the highest signal‐to‐background ratio, due to a very low electrochemical background level. When EN redox cycling was applied to a sandwich‐type immunosensor, parathyroid hormone (PTH) in serum could be detected with a very low detection limit of ∼0.1 pg/mL.