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Improved Sensing Performance of Amperometric Urea Biosensor by Using Platinum Nanoparticles
Author(s) -
Korkut Uru Seyda,
Samet Kilic Muhammet,
Yetiren Fatma
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202100071
Subject(s) - amperometry , urease , urea , biosensor , platinum nanoparticles , platinum , chemistry , electrode , nuclear chemistry , chromatography , electrochemistry , catalysis , organic chemistry , biochemistry
A novel Platinum nanoparticle (PtNPs) modified Poly(pyrrole‐ co ‐1‐(2‐Aminophenyl)pyrrole)/Urease film coated Au electrode was designed for amperometric detection of urea. PtNPs quantity, film density and pH were optimized and interference effect of some substances readily found in municipal wastewater and blood was investigated. The biosensor responded to urea with a measurement concentration range of 0.1 to 30 mM, a sensitivity of 31.8 μA mM −1 cm −2 , a LOD of 7.58 μM, an accuracy of 104 % and a RSD% of only 0.82. It sensed the concentration of urea in the municipal sewage water with recovery of 97.6 % (n=3) and remained 78 % of its initial response at 28 th day. Results confirmed that PtNPs with strong conductivity improved the electron transfer ability of the working electrode.