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Electrochemical Aptasensor for Zearalenone Based on DNA Assembly and Exonuclease III as Amplification Strategy
Author(s) -
Wei Min,
Xin Lingkun,
Jin Huali,
Huang Yawei,
Liu Yong
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202100043
Subject(s) - zearalenone , ochratoxin a , detection limit , aflatoxin , reproducibility , linear range , chemistry , chromatography , exonuclease , repeatability , mycotoxin , exonuclease iii , dna , food science , biochemistry , escherichia coli , gene , dna polymerase
A sensitively electrochemical aptasensor was developed to detect zearalenone, utilizing DNA assembly based on hybridization chain reaction to amplify the signal current and exonuclease III to reduce the background current. The linear range 5.0×10 −5 ng/mL‐50 ng/mL, and the limit of detection is 0.013 pg/mL. The fabricated aptasensor showed the high specificity toward aflatoxin B1 (AFB1), fumonisin B1 (FB1) and ochratoxin A (OTA), good repeatability and reproducibility. In addition, the average recoveries of spiked corn and beer samples were in the range of 89 % to 102 %. The established method is of great significance in the field of food safety detection.