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Electrochemical Enhanced Detection of Uric Acid Based on Peroxidase‐like Activity of Fe 3 O 4 @Au
Author(s) -
Guan Huanan,
Peng Bo,
Gong Dezhuang,
Han Bolin,
Zhang Na
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202100036
Subject(s) - detection limit , electrochemistry , uric acid , catalysis , electrode , selectivity , reproducibility , chemistry , materials science , electrochemical gas sensor , chromatography , nuclear chemistry , biochemistry
Fe 3 O 4 @Au nanomaterials were prepared by the self‐assembly method. An enzyme‐free, ultrasensitive electrochemical detection of uric acid was achieved based on the peroxidase‐like activity of Fe 3 O 4 @Au. The proposed procedure has exhibited excellent catalytic activities and achieved significant enhancements of the current responses to uric acid. The detection range was from 0.1 to 10 mmol/L, and the limit of detection was 0.087 μmol/L. Under the action of external magnetic field, the magnetic particles can be easily separated from the bottom liquid, which has the advantages of simple operation and high separation efficiency. Moreover, this detection method combining a simulated enzyme and electrochemical can enhance the effective output of the overall electrochemical signal without modifying the electrode, and excellent reproducibility can be achieved. Compared to colorimetric assay, the electrochemical one has higher sensitivity and selectivity, and was further applied in ultrasensitive detection of uric acid in food samples. In short, the proposed electrochemical assay has great potential in the fields of food quality control and biomedical analysis.