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An Ultra‐sensitive Electrochemiluminescent Detection of Carcinoembryonic Antigen Using a Hollowed‐out Electrode
Author(s) -
Liu Gen,
Gao Hui,
Chen Jiajia,
Shao Congying,
Chen Feifei
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060624
Subject(s) - carcinoembryonic antigen , detection limit , electrochemiluminescence , electrode , reproducibility , carbon nanotube , linear range , materials science , nanotechnology , analytical chemistry (journal) , chromatography , chemistry , cancer , medicine
Ultrasensitive detection of cancer biomarkers has attracted considerable attention recently in academic research and clinic diagnostics. Here, we use a hollowed‐out carbon nanotube sponge (CNTSP) electrode to fabricate an immunosensor to realize the sensitive detection of carcinoembryonic antigen (CEA). Nitrogen‐doped carbon quantum dots (N‐CDs) are combined with antibody that can specially recognize CEA and used as the electrochemiluminescent (ECL) probes in this work. The hollowed‐out and permeable CNTSP facilitates chemical species exchange on the surface of electrode, offering an enhanced ECL signal. The resulting ECL immunosensor enables the determination of CEA concentration to be in a wide linear range from 0.005 to 50 pg mL −1 with a detection limit of 1.4 fg mL −1 . Furthermore, with good stability, acceptable precision and reproducibility, the proposed ECL assay strategy offers a wide application potential in clinical analysis.