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A Novel GABA B R1a Receptor Electrochemical Biosensor Based on Gold Nanoparticles Chitosan‐horseradish Peroxidase
Author(s) -
Zhao Xiaotong,
Wei Lihui,
Pang Guangchang,
Xie Junbo
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060594
Subject(s) - horseradish peroxidase , biosensor , chitosan , chemistry , electrochemistry , colloidal gold , nanoparticle , peroxidase , combinatorial chemistry , nuclear chemistry , nanotechnology , chromatography , biochemistry , enzyme , materials science , electrode
A novel gamma aminobutyric acid type B receptor subunit 1a (GABA B R1a) biosensor modified with double‐layer gold has been developed for the first time. In this work, the synthesized plasmid was transfected into HEK‐293T cells by liposome transfection, and the target protein GABA B R1a was obtained. The horseradish peroxidase was used as a signal amplification system to produce a double‐layer gold modified GABA B R1a receptor biosensor. The ligand gamma aminobutyric acid (GABA), jujuboside A and baclofen were detected by the time‐current method with the biosensor. The results showed that the action regularity of GABA, jujuboside A, and baclofen on GABA B R1a receptor was fitted with hyperbolic fitting (R 2 was 0.9740, 0.9770 and 0.9770, respectively). The double reciprocal method was used to figure out the affinity constant (K a ) of GABA, jujuboside A, and baclofen with the receptor, i. e. 2.1016×10 −15 mol/L, 1.7601×10 −14 mol/L, and 1.633×10 −14 mol/L, respectively, indicating that these measured compounds exhibited different action strength on the receptor. Based on the above, this study will provide a basis for drug screening and will also provide a new idea and method for investigating quantitatively the interaction between receptors and ligands in the future.